So here's a little description of what I believe explains the difference between&Delta G and &Delta G0.Gibb's Free Energy 

Biochemistry Class Notebook

Thermodynamics....huh?

Here is a quick review of pH, pK and pI that will be important when we begin Chapter 4: amino acids.Amino Acid Titration.For Window Media Players 

pH and Amino Acid Titration

Here is a summary of some important details concerning amino acid structure and properties. You should also be able to download theses videos for free from the iTunes music store if you have an account. You will find it in the Podcast section under "Biochemistry Class Notebook". Stevie...what more can I say. 

Chapter 4: Amino Acids

Here's a brief overview of the most challenging topic in chapter 7, The Bohr Effect. 

The Bohr Effect

Here is the first of 2 installments on our Enzyme Kinetics lectures. Use this for a quick review of the terms KM, VMAX, Kcat, KS and enzyme efficiency. More description of the Linweaver-Burke and inhibition will follow in the 2nd installment. 

Michaelis-Menton Kinetics after Exam II

Hope you listen in on this description of the 3 common enzyme inhibition profiles, it may prove helpful on Exam III. 

Enzyme Inhibition

(click the title to play or right click it to download and play in Quicktime)The first experiment in Biochemistry lab this coming week is the Spectrophotometry of Riboflavin. Take a look at the Spectrophotometry of Riboflavin, Part I before coming to class.If you're having trouble downloading and viewing the link on the title then this link will redirect you to the source file and you should be able to hear and see it there. 

BIOCHEM LAB: Spectrophotometry of Riboflavin Part I

(click the title to play or right click to download and play in Quicktime)The first experiment in Biochemistry lab this coming week is the Spectrophotometry of Riboflavin. Take a look at the Spectrophotometry of Riboflavin, Part II video before class.If you're having trouble downloading and viewing the link on the title then this link will redirect you to the source file and you should be able to hear and see it there. 

BIOCHEM LAB: Spectrophotometry of Riboflavin Part II

Read your manual and do your calculations before class. Let me know if you have trouble viewing the Quicktime movie. 

BIOCHEM LAB: pH Determination

The amino acid titration experiment is described in this 20 minute video. CLICK the title to download and play a windows media file (not very good). Here you can download and play the quicktime file or get it from iTunes. Finally to accommodate all of you you may be able to view this video below. Go through it before taking the bonus quiz. 

BIOCHEM LAB: Titration of an Amino Acid

In the Experiment IV video will take a stock solution of BSA to generate a standard curve to use to determine the concentration of an unknown concentration of BSA. BSA is a standard size protein and is used regularly as a control to compare and determine concentrations of many different types of pure proteins. Can you tell me what BSA stands for? 

Protein Concentration Determination

Experiment VI and part of Experiment VII are described in this video. There is forthcoming the actual loading, running, staining and destaining of the gel before the final so stay tuned. You can access this video from iTunes or click the title here and view it or download it from my archives if having trouble viewing here. It may be necessary to wait until the video completes buffering to get a smooth view. 

Protein Characterization and PAG Electrophoresis

Post comments, criticisms and questions you may have in Biochemistry to help this blog develop. Use this as often as you need. The more we communicate...the more you learn. This material assumes an advanced understanding of biology and chemistry as all of these videos are created after the actual lecture presentations on the topics. Send me your comments!!

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Biochemistry Class Notebook

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